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Alphapithovirus is a genus of giant virus known from two species, Alphapithovirus siberiense, which infects amoebae, and Alphapithovirus massiliense. It is DNA-based and is a member of the nucleocytoplasmic large DNA viruses clade. It was discovered in 2014, when a viable specimen was found in a 30,000-year-old ice core harvested from permafrost in Siberia, Russia. It is the third-largest known giant virus, behind only Megaklothovirus horridgei and Klothovirus casanovai.
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