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DNA amplification fingerprinting (DAF) is a highly sensitive DNA profiling technique that generates complex, reproducible genomic fingerprints without prior knowledge of sequence information. Developed in the very early 1990s by Gustavo Caetano-Anollés and colleagues at the University of Tennessee, DAF offered a high-resolution alternative to nucleic acid scanning methods such as random amplified polymorphic DNA (RAPD), arbitrarily primed PCR (AP-PCR), and amplified fragment length polymorphism (AFLP) for genetic typing, strain discrimination, genome mapping, and population analysis.
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