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  • Date: 2026-04-02 05:42:49

This topic has appeared in the trending rankings 1 time(s) in the past year. While it does not trend frequently, its appearance suggests a renewed or concentrated surge of public interest.

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Eagle's minimal essential medium

Wikipedia Overview

Minimal essential medium (MEM) is a synthetic cell culture medium developed by Harry Eagle first published in 1959 in Science that can be used to maintain cells in tissue culture. It is based on six salts and glucose described in Earle's salts in 1943: calcium chloride, potassium chloride, magnesium sulfate, sodium chloride, sodium phosphate and sodium bicarbonate. The medium is further supplemented with thirteen essential amino acids, and eight vitamins: thiamine (vitamin B1), riboflavin (vitamin B2), nicotinamide (vitamin B3), pantothenic acid (vitamin B5), pyrodoxine (vitamin B6), folic acid (vitamin B9), choline, and myo-inositol (originally known as vitamin B8). Many variations of this medium have been developed, mostly adding additional vitamins, amino acids, and/or other nutrients.

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