Why is "Periodic acid–Schiff stain" trending?
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- Ranking position: #
- Date: 2026-04-03 06:44:16
This topic has appeared in the trending rankings 1 time(s) in the past year. While it does not trend frequently, its appearance suggests a renewed or concentrated surge of public interest.
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Wikipedia Overview
Periodic acid–Schiff (PAS) is a staining method used to detect polysaccharides and mucosubstances in tissues. The reaction of periodic acid oxidizes vicinal diols in these sugars, usually breaking up the bond between two adjacent carbons not involved in the glycosidic linkage or ring closure in the ring of monosaccharide units that are part of the long polysaccharides and creating a pair of aldehydes at the two free tips of each broken monosaccharide ring. The oxidation condition has to be sufficiently regulated so as to not further oxidize the aldehydes. These aldehydes then react with the Schiff reagent to give a purple-magenta color. A suitable basic stain is often used as a counterstain.
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